Acid Cellulase CAS:9012-54-8 SUKACell INTRODUCTION Cellulose is an unbranched glucose polymer composed of anhydro-D-glucose units linked by 1,4-b-D-glucoside bonds. These glycosidic bonds can be hydrolysed by cellulolytic enzymes. The native structure of the cellulose is composed of crystalline and less organized amorphous regions. DESCRIPTION SUKACell is an enzyme preparation obtained from the submerged fermentation of a high cellulase-producing microbial strain. It is widely used in feed, textile, wastewater treatment and paper making industries. WORKING MECHANISM The 1,4-b-D-glucosidic linkages in cellulose, lichenin and cereal b-D glucans are hydrolyzed to release free glucose units through the successive action of cellulase enzymes. The less organized amorphous region at the center of the cellulose chain is initially attacked by endoglucanase by random cutting of the b-1,4 glycosidic linkages within the chain thereby producing cello-oligosaccharides. Cellobiohydrolases I and II act on the cello-oligosaccharides from the reducing end and non-reducing end of the cello-oligosaccharides to release cellobiose. b-glucosidase act on the released cellobiose to produce b–D glucose units. PROPERTIES SUKACell provides maximal benefit at pH 4.8 – 5.2 and at the temperature of 55 – 60℃ (131 – 140℉). SPECIFICATION SUKACell is available in liquid preparation as: SUKACell LQ10 (with enzyme activity of 10,000 U/mL) SUKACell LQ20 (with enzyme activity of 20,000 U/mL) SUKACell is available in solid preparation as: SUKACell PW10 (with enzyme activity of 10,000 U/g) SUKACell PW20 (with enzyme activity of 20,000 U/g) Unit definition: The amount of enzyme needed to produce 1 mg glucose from carboxymethylcellulose (CMC) in one hour at specified conditions by the DNS method. APPLICATION GUIDE ? For use as a feed additive, SUKACell can be added to the mix at a rate of 0.05-0.1%. ? For use in the textile industry, add SUKACell at the rate of 2.5-5 g/kg of fabric dissolved as 5:1 water : clothes ratio. The product can also be added at 5-10 g/kg of fabric dissolved as 10:1 water : clothes ratio. ? For the paper making industry, SUKACell is added based on the instructions of the technicians. PACKAGING SUKACell is packaged in 25 kg/plastic drum and 1000 L/plastic drum for the liquid form while the solid preparation is packaged as 20 kg per box or film lined woven bag. Alternative packaging is available upon request for smaller or larger volumes. STORAGE Store in a cool, dry place and away from direct sunlight. SUKACell maintains its activity for considerably longer time when stored at lower temperatures (below 25℃). The product should not be frozen. HANDLING PRECAUTIONS SUKACell is non-toxic and biodegradable. However, unnecessary contact with the product should be avoided. Long term exposure to protein based products such as SUKACell may sensitize certain individuals to the product. Wash hands with warm soapy water after handling. Keep out of reach of children.
feed additive, textile enzyme